| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stewart, Sydni R (16)
|
MASAKA |
24.29 |
24.60 |
0.31 |
| 2 |
Bowne, Jamie O (16)
|
MAGPAC |
24.80 |
25.31 |
0.51 |
| 3 |
Hallam, Kyla M (16)
|
MAGPAC |
|
25.57 |
- |
| 4 |
Wakeley, Laura C (15)
|
MAGPAC |
26.15 |
25.97 |
-0.18 |
| 5 |
Washart, Jordan L (15)
|
MAGPAC |
26.29 |
26.15 |
-0.14 |
| 5 |
Copeland, Brittany M (26)
|
MASAKA |
25.64 |
26.15 |
0.51 |
| 7 |
Parker, Nia C (16)
|
MASAKA |
26.59 |
26.38 |
-0.21 |
| 8 |
Venit, Veronica (17)
|
MAGPAC |
27.11 |
27.16 |
0.05 |
| 9 |
Williams, Gabrielle A (15)
|
MAUN |
|
27.67 |
- |
| 10 |
Jackson, India (15)
|
MASAKA |
27.29 |
27.75 |
0.46 |
| 11 |
Bedesem, Caitlin (17)
|
MANRG |
26.79 |
27.90 |
1.11 |
| 12 |
Taylor, Kaia T (15)
|
MASERP |
28.55 |
29.19 |
0.64 |